HPLC simulation
and teaching resources

Browser-based tools for studying high-performance liquid chromatography. Calculate chromatograms, draw flow paths, and examine how operating conditions affect a separation.

HPLC Simulator gradient-elution chromatogram with numbered peaks, a red selected-compound trace, and increasing solvent B on the right axis.

Applications

Both applications run in the browser without Java.

0246810Time (min)Detector response
Chromatographic separations

HPLC Simulator

Calculate chromatograms for isocratic and gradient runs. Adjust mobile-phase composition, flow rate, column properties, and sample composition to compare retention times, peak widths, and resolution.

Open HPLC Simulator
SolventPumpInjectorColumnDetector
System plumbing

HPLC Fluid Visualizer

Draw flow paths using pumps, valves, tubing, columns, and detectors. Calculate system backpressure, band broadening, and gradient delay for the connected components.

Open HPLC Fluid Visualizer

Educational resources

Download contributed problem sets, simulator exercises, instructor notes, and a companion spreadsheet.

View resources